VOLUME XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)

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"This volume contains a broad selection of techniques that should be useful to those interested in studying the cyclic nucleotide content of intact cells and the biosynthesis, degradation, and action of cyclic nucleotides in cell-free systems. Techniques for purifying and assaying a single enzyme or nucleotide will be described in more than one article. This is largely by editorial design, not to compile a complete collection of methods, but to offer the investigator a choice of useful techniques. All of them will be reliable when properly used, but none of them will be, for all laboratories, either easier or more reliable to use than any of the others. In some cases (as, for example, with adenylate cyclase from mammalian sources) lability or lack of extensive purification of an enzyme has contributed to technical problems that differ from tissue to tissue. In other cases (as, for example, with cyclic nucleotide phosphodiesterase) certain properties of an enzyme will be found to be distinctly different from tissue to tissue or among multiple forms of the enzyme from a single tissue."