VOLUME XL. Hormone Action (Part E: Nuclear Structure and Function)

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"In this volume, we have attempted to collect a series of methodologies which would allow investigators to assess hormone effects on nuclear structure, DNA replication, mitosis, and gene transcription. Techniques for assessing nucleic acid metabolism per se are conspicuously absent from this volume but are thoroughly covered in other volumes of "Methods in Enzymology." In considering gene function and specific gene restriction in eukaryotes, it seems almost certain that these processes will ultimately be related to the composition and location of chromosomal proteins. For this reason, we have emphasized techniques which can be utilized to study chromatin composition, structure, and function. This volume also contains a collection of general techniques and analytical approaches important to those involved in the study of hormone action but which have not been included in the earlier volumes of this series because they did not fall clearly within the previously defined categories."