VOLUME XXII. Enzyme Purification and Related Techniques
Available here.
"Aside from methods related directly to purification, this volume includes a description of peripheral techniques of value in enzyme preparation. For example, much space has been allowed for the methodology of obtaining cells which must be grown in the laboratory, and emphasis has been placed on the isolation of specialized organelles. Many useful general methods which have been included in previous volumes of this series are listed in the section to which they apply. Such a list is not only particularly useful as a source of direction for isolating specific organelles but also explains why a separate article was not included for the single, most common means of enzyme purification, salting out. The latter was so well recorded in a previous volume that reference to the article should suffice."