VOLUME V. Preparation and Assay of Enzymes

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Section I. General Preparative Procedures

1. Column Chromatography of Proteins.
2. Preparative Electrophoresis.
3. Preparation and Solubilization of Particles (Bacterial, Mammalian, and Higher Plant).
4. Mammalian Cell Culture.
5. Protoplasts.

Section II. Enzymes of Carbohydrate Metabolism

1. Polysaccharide Cleavage and Synthesis.
2. Disaccharide, Hexoside and Glucuronide Metabolism.
3. Metabolism of Hexoses, Pentoses, and 3-Carbon Compounds.
4. Hexosamine and Sialic Acid Metabolism.
5. Aromatic Ring Synthesis.

Section III. Enzymes of Lipid Metabolism

1. Fatty Acid Synthesis and Breakdown.
2. Acid Activating Enzymes.
3. Phospholipid Synthesis and Breakdown.
4. Steroid Metabolism.

Section IV. Enzymes of Citric Acid Cycle

1. Krebs Cycle.
2. Krebs-Kornberg Cycle.
3. Related Enzymes.

Section V. Enzymes of Protein Metabolism

1. Proteolytic Enzymes.
2. Amino Acid Dehydrogenases and Transaminases.
3. Amino Acid Activating Enzymes.
4. Other Enzymes of Amino Acid Breakdown and Synthesis.
5. Enzymes of Sulfur Metabolism.