VOLUME XIII. Citric Acid Cycle

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"The citric acid cycle represents the terminal stage for the oxidation of the major foodstuffs and energy stores in many organisms. In addition, the cycle plays an important part in the synthesis of many cell constituents from simple precursors. This volume of "Methods in Enzymology" deals with the reactions of the citric acid cycle and with a number of reactions leading to and from the cycle. In deciding what to include in the present volume, boundaries had to be drawn between various areas of metabolism and the citric acid cycle. These boundaries are of necessity somewhat arbitrary. The aspartase reaction is included. On the other hand, enzymes which might have been included here, such as certain transaminases and glutamate dehydrogenase, will be covered in a future volume devoted to amino acid metabolism. A few of the methods which are included should logically have appeared in earlier volumes. These are warranted by the importance of the topic and the time it would take for them to appear in future revisions of recent volumes."