VOLUME XXVII. Enzyme Structure (Part D)

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"This is the second of two volumes of "Enzyme Structure" devoted to physical methods. (Part C, Volume 26 of "Methods in Enzymology," appeared recently.) Although coverage of the various techniques is not exhaustive, it is hoped that the intent of presenting a broad coverage of currently available methods has been reasonably fulfilled."

"These volumes present not only techniques that are currently widely available but some which are only beginning to make an impact and some for which no commercial standard equipment is as yet available. In the latter cases, an attempt has been made to guide the reader in assembling his own equipment from individual components and to help him find the necessary information in the research literature."

"In the coverage of physical techniques, we have departed somewhat in scope from the traditional format of the series. Since, at the termination of an experiment, physical techniques frequently require much more interpretation than do organic ones, we consider that brief sections on the theoretical principles involved are highly desirable as are sections on theoretical and mathematical approaches to data evaluation and on assumptions and, consequently, limitations involved in the applications of the various methods."

"The division of the material between the two parts is arbitrary. Thus, there is a considerable amount of overlap between general categories, and, at times, the descriptions of closely related techniques are found divided between Parts C and D. We do not believe, however, that this should hinder the reader in his use of these volumes for, in every case, each chapter is a completely self-contained unit."