Difference between revisions of "Zinc-fingers"
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| − | Zinc Finger Nucleases (ZFNs) were the first widely used programmable DNA binding protein system. ZFNs are comprised of a chain of zinc finger proteins fused to a bacterial nuclease to produce a system capable of making site-specific double stranded DNA breaks to enable gene edits. The zinc finger proteins provide site specific targeting as they each recognize a 3–4 base pair DNA sequence. | + | ==Overview== |
| + | Zinc Finger Nucleases (ZFNs) were the first widely used programmable DNA binding protein system. ZFNs are comprised of a chain of zinc finger proteins fused to a bacterial nuclease to produce a system capable of making site-specific double stranded DNA breaks to enable gene edits. The zinc finger proteins provide site specific DNA targeting as they each recognize a 3–4 base pair DNA sequence. | ||
| − | In ZFNs, a chain of zinc finger proteins are utilized to recognize a longer, more specific locus within the genome. The nuclease commonly used in ZFN technology, fused to this chain of zinc finger proteins, is FokI, which must dimerize in order to introduce a DSB. | + | In ZFNs, a chain of zinc finger proteins are utilized to recognize a longer, more specific locus within the genome. The nuclease commonly used in ZFN technology, fused to this chain of zinc finger proteins, is FokI, which must dimerize in order to introduce a DSB. Therefore a pair of ZFNs are used together to target and cut the DNA. |
| + | ==Overview Figure== | ||
| + | ==Details== | ||
Latest revision as of 20:41, 9 September 2021
Overview
Zinc Finger Nucleases (ZFNs) were the first widely used programmable DNA binding protein system. ZFNs are comprised of a chain of zinc finger proteins fused to a bacterial nuclease to produce a system capable of making site-specific double stranded DNA breaks to enable gene edits. The zinc finger proteins provide site specific DNA targeting as they each recognize a 3–4 base pair DNA sequence.
In ZFNs, a chain of zinc finger proteins are utilized to recognize a longer, more specific locus within the genome. The nuclease commonly used in ZFN technology, fused to this chain of zinc finger proteins, is FokI, which must dimerize in order to introduce a DSB. Therefore a pair of ZFNs are used together to target and cut the DNA.
